沙门菌感染雏鸡调节性T细胞变化的分析

doi:10.11843/j.issn.0366-6964.2019.11.014

摘要/Abstract

摘要： 旨在分析雏鸡沙门菌感染后其脾调节性T细胞（Tregs）比例变化。本研究以京星黄鸡H系为试验群，将1日龄雏鸡随机分为对照组（n=40）和感染组（n=80），相同环境无菌饲养。7日龄时，感染组接种沙门菌建立感染模型，对照组以生理盐水代替沙门菌。感染48 h后，分别对两组雏鸡盲肠扁桃体免疫反应相关基因表达水平、血清炎症因子进行测定，并分别从中随机抽取雏鸡（对照组n=25，感染组n=34）检测其脾中CD4⁺T细胞、Tregs/CD4⁺T细胞比例。结果显示：感染组与对照组相比，脾中CD4⁺T细胞比例无显著性差异（P>0.05）。感染组与对照组CD4⁺CD25⁺/CD4⁺（%）分别为0.93±0.12与3.22±0.59（P<0.01）；CD4⁺TGF-β⁺/CD4⁺（%）分别为0.55±0.07与1.42±0.25（P<0.01）；CD4⁺CD25⁺TGF-β⁺/CD4⁺（%）分别为0.29±0.04与0.76±0.14（P<0.01），均呈现显著性差异。雏鸡感染沙门菌后，脾中CD4⁺T细胞比例无显著性差异，而Tregs/CD4⁺T细胞比例较对照组明显下降（P<0.01）。本研究提示调节性T细胞可能参与雏鸡沙门菌感染的发病过程，为进一步研究雏鸡抗沙门菌感染机制提供理论依据。

Abstract: The objective of this study was to analyze the proportion of spleen regulatory T cells (Tregs) in chicks after Salmonella infection. In this study, Jing xing yellow chicken H lines were used as experimental groups, and 1-day-old chicks were randomly divided into control group (n=40) and infected group (n=80).Animals were kept in the same and sterile environment. At 7 days of age, the infected group was infected with Salmonella (Salmonella typhimurium CICC 21484) to establish an infection model. The control group was treated by saline instead of Salmonella. After 48 hours of infection, the expression levels of cecal tonsil immune response-related genes and serum inflammatory factors were measured in two groups. Then, two groups of chicks were randomly selected (control group, n=25; infected group, n=34) to detect CD4⁺ T cells and Tregs/CD4⁺T cells ratio of spleens. The results showed that there was no significant difference in the proportion of CD4⁺T cells in the spleens between control gruop and infected group (P>0.05). In infected group and control group, CD4⁺ CD25⁺/CD4⁺T cells (%) were 0.93±0.12 and 3.22±0.59, respectively (P< 0.01); CD4⁺ TGF-β⁺/CD4⁺T cells (%) were 0.55±0.07 and 1.42±0.25 (P<0.01), respectively; CD4⁺CD25⁺ TGF-β⁺/CD4⁺T cells (%) were 0.29±0.04 and 0.76±0.14, respectively (P<0.01), there were significant differences between the above data of the two groups. There was no significant difference in the proportion of CD4⁺T cells in the spleens of chicks after Salmonella infection. The ratio of Tregs/CD4⁺T cells were significantly lower than that in the control group (P<0.01). It is suggested that Tregs may be involved in the pathogenesis of Salmonella infection in chicks, which provides a theoretical basis for further study on the mechanism of chicks against Salmonella infection.

中图分类号:

S852.4

张锦, 李庆贺, 郑麦青, 刘冉冉, 崔焕先, 文杰, 赵桂苹. 沙门菌感染雏鸡调节性T细胞变化的分析[J]. 畜牧兽医学报, 2019, 50(11): 2302-2308.

ZHANG Jin, LI Qinghe, ZHENG Maiqing, LIU Ranran, CUI Huanxian, WEN Jie, ZHAO Guiping. Analysis of Regulatory T Cells Changes in Chicks Infected with Salmonella[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(11): 2302-2308.

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参考文献 30

[1]	PASSERINI L, DI NUNZIO S, GREGORI S, et al. Functional type 1 regulatory T cells develop regardless of FOXP3mutations in patients with IPEX syndrome[J]. Eur J Immunol, 2011, 41(4):1120-1131.
[2]	CARRIER Y, JING Y, KUCHROO V K, et al. Th3 cells in peripheral tolerance. I. Induction of Foxp3-positive regulatory T cells by Th3 cells derived from TGF-β T cell-transgenic mice[J]. J Immunol, 2007, 178(1):179-85.
[3]	LU L R, CANTOR H. Generation and regulation of CD8+ regulatory T cells[J]. Cell Mol Immunol, 2008, 5(6):401-406.
[4]	HOFFMANN J C, PAWLOWSKI N N, GROLLICH K, et al. γδ T lymphocytes:a new type of regulatory T cells suppressing murine 2, 4, 6-trinitrobenzene sulphonic acid (TNBS)-induced colitis[J]. Int J Color Dis, 2008, 23(10):909-920.
[5]	SMYTH M J, GODFREY D I. NKT cells and tumor immunity-a double-edged sword[J]. Nat Immunol, 2000, 1(6):459-460.
[6]	FONTENOT J D, GAVIN M A, RUDENSKY A Y. Foxp3 programs the development and function of CD4+CD25+ regulatory T cells[J]. Nat Immunol, 2003, 4(4):330-336.
[7]	HORI S, NOMURA T, SAKAGUCHI S. Pillars article:control of regulatory T cell development by the transcription factor Foxp3. Science 2003. 299:1057-1061[J]. J Immunol, 2017, 198(3):981-985.
[8]	ANDERSEN K G, NISSEN J K, BETZ A G. Comparative genomics reveals key gain-of-function events in Foxp3 during regulatory T cell evolution[J]. Front Immunol, 2012, 3:113.
[9]	SHANMUGASUNDARAM R, SELVARAJ R K. CD4+CD25+ regulatory T cell ontogeny and preferential migration to the cecal tonsils in chickens[J]. PLoS One, 2012, 7(3):e33970.
[10]	GURUNG A, KAMBLE N, KAUFER B B, et al. Association of Marek's disease induced immunosuppression with activation of a novel regulatory T cells in chickens[J]. PLoS Pathog, 2017, 13(12):e1006745.
[11]	REVOLLEDO L, FERREIRA A J P. Current perspectives in avian salmonellosis:vaccines and immune mechanisms of protection[J]. J Appl Poultry Res, 2012, 21(2):418-431.
[12]	FOLEY S L, LYNNE A M. Food animal-associated Salmonella challenges:pathogenicity and antimicrobial resistance[J]. J Anim Sci, 2008, 86(14 Suppl):E173-E187.
[13]	GANTOIS I, DUCATELLE R, PASMANS F, et al. Mechanisms of egg contamination by Salmonella enteritidis[J]. FEMS Microbiol Rev, 2010, 33(4):718-738.
[14]	KAWAI T, AKIRA S. The role of pattern-recognition receptors in innate immunity:update on Toll-like receptors[J]. Nat Immunol, 2010, 11(5):373-384.
[15]	ZHANG A G, XU J H, LAI H Z, et al. Age-related changes and distribution of T cell markers (CD3 and CD4) and toll-like receptors(TLR2, TLR3, TLR4 and TLR7) in the duck lymphoid organs[J]. Immunobiology, 2017, 222(7):857-864.
[16]	LI H L, JIN H, LI Y Q, et al. Molecular cloning and functional characterization of duck nucleotide-binding oligomerization domain 1(NOD1)[J]. Dev Comparat Immunol, 2017, 74:82-89.
[17]	DIEHL S, ANGUITA J, HOFFMEYER A, et al. Inhibition of Th1 differentiation by IL-6 is mediated by SOCS1[J]. Immunity, 2000, 13(6):805-815.
[18]	CHOMARAT P, BANCHEREAU J, DAVOUST J, et al. IL-6 switches the differentiation of monocytes from dendritic cells to macrophages[J]. Nat Immunol, 2000, 1(6):510-514.
[19]	RIAZI K, GALIC M A, KUZMISKI J B, et al. Microglial activation and TNFα production mediate altered CNS excitability following peripheral inflammation[J]. Proc Natl Acad Sci U S A, 2008, 105(44):17151-17156.
[20]	LAI J L, LIU Y H, LIU C, et al. Indirubin inhibits LPS-induced inflammation via TLR4 abrogation mediated by the NF-κB and MAPK signaling pathways[J]. Inflammation, 2017, 40(1):1-12.
[21]	FIORENTINO D F, ZLOTNIK A, MOSMANN T R, et al. IL-10 inhibits cytokine production by activated macrophages[J]. J Immunol, 1991, 147(11):3815-3822.
[22]	ELSON C O, CONG Y Z, IQBAL N, et al. Immuno-bacterial homeostasis in the gut:new insights into an old enigma[J]. Semin Immunol, 2001, 13(3):187-194.
[23]	ISOLAURI E, MAJAMAA H, ARVOLA T, et al. Lactobacillus casei strain GG reverses increased intestinal permeability induced by cow milk in suckling rats[J]. Gastroenterology, 1993, 105(6):1643-1650.
[24]	GHEBREMICAEL S B, HASENSTEIN J R, LAMONT S J. Association of interleukin-10 cluster genes and Salmonella response in the chicken[J]. Poultry Sci, 2008, 87(1):22-26.
[25]	LILLEHOJ H S, LILLEHOJ E P. Avian coccidiosis. A review of acquired intestinal immunity and vaccination strategies[J]. Avian Dis, 2000, 44(2):408-425.
[26]	KINTER A L, HENNESSEY M, BELL A, et al. CD25+CD4+ regulatory T cells from the peripheral blood of asymptomatic HIV-infected individuals regulate CD4+ and CD8+ HIV-specific T cell immune responses in vitro and are associated with favorable clinical markers of disease status[J]. J Exp Med, 2004, 200(3):331-343.
[27]	吕凌,张峰,王学浩,等. 大鼠CD4+CD25+T调节细胞的分离培养及其功能分析[J]. 细胞与分子免疫学杂志, 2006, 22(4):417-419.
	LÜ L, ZHANG F, WANG X H, et al. Isolation and function analysis of rat CD4+CD25+ regulatory T cells[J]. Chinese Journal of Cellular and Molecular Immunology, 2006, 22(4):417-419. (in Chinese)
[28]	MADDEN J A J, HUNTER J O. A review of the role of the gut microflora in irritable bowel syndrome and the effects of probiotics[J]. Br J Nutr, 2002, 88(Suppl 1):S67-S72.
[29]	JOHANNS T M, ERTELT J M, ROWE J H, et al. Regulatory T cell suppressive potency dictates the balance between bacterial proliferation and clearance during persistent Salmonella infection[J]. PLoS Pathog, 2010, 6(8):e1001043.
[30]	SHANMUGASUNDARAM R, SELVARAJ R K. Regulatory T cell properties of chicken CD4+CD25+ cells[J]. J Immunol, 2011, 186(4):1997-2002.